![]() Genome-wide analysis of the bZIP gene family identifies two ABI5-like bZIP transcription factors, BrABI5a and BrABI5b, as positive modulators of ABA signalling in Chinese cabbage. The Phaseolus vulgaris ZIP gene family: identification, characterization, mapping, and gene expression. The objective of this research was to clone the peroxidase genes homologs in cassava variety Rayong 60 (MTAI 8), which is a disease-resistant variety against cassava bacterial blight by using polymerase chain reaction method. Differential expression of four soybean bZIP genes during Phakopsora pachyrhizi infection. Plant bZIP transcription factors responsive to pathogens: a review. Genome wide identification of orthologous ZIP genes associated with zinc and iron translocation in Setaria italica. Taken together, this study highlights the positive role of MebZIP3 and MebZIP5 in disease resistance against cassava bacterial blight for further utilization in genetic improvement of cassava disease resistance.īasic leucine zipper (bZIP) transcription factor cassava (Manihot esculenta) cassava bacterial blight disease resistance virus-induced gene silencing (VIGS).Īlagarasan G., Dubey M., Aswathy K. On the contrary, MebZIP3- and MebZIP5-silenced plants by virus-induced gene silencing (VIGS) showed disease sensitive phenotype, lower transcript levels of defense-related genes and less callose depositions. Through overexpression in tobacco, we found that MebZIP3 and MebZIP5 conferred improved disease resistance against cassava bacterial blight, with more callose depositions. Subcellular localization analysis showed that MebZIP3 and MebZIP5 are specifically located in cell nucleus. manihotis ( Xam), salicylic acid (SA), and hydrogen peroxide (H 2O 2). Gene expression analysis indicated that MebZIP3 and MebZIP5 were commonly regulated by flg22, Xanthomonas axonopodis pv. ![]() ABSTRACT Cassava suffers from bacterial blight attack in all growing regions. In this study, we analyzed the expression pattern and the function of two MebZIPs ( MebZIP3 and MebZIP5) in response to pathogen infection. The quantitative trait loci (QTL) mapping results suggest that the four. Although 77 MebZIPs have been genome-wide identified in cassava, their in vivo roles remain unknown. A scheme to confirm the usefulness of these markers in evaluating segregating populations for resistance to CBB is proposed.Basic domain-leucine zipper (bZIP) transcription factor, one type of conserved gene family, plays an important role in plant development and stress responses. With the male-derived framework map, four QTLs on linkage groups G and C explained 10.7–27.1% of the variance. Based on the AUDPC values, eight QTLs (quantitative trait loci), located on linkage groups B, D, L, N, and X of the female-derived framework map, were found to explain 9–20% of the phenotypic variance of the crop’s response to the five Xam strains. Area under the disease progress curve (AUDPC) was used as a quantitative measure of resistance in QTL analysis by single-marker regression. Five Xam strains were used: CIO-84, CIO-1, CIO-136, CIO-295, and ORST X-27. Major Novel QTL for Resistance to Cassava Bacterial Blight Identified through a Multi-Environmental Analysis Front Plant Sci. To better understand the genetics of resistance to CBB, we evaluated individuals of the F 1 cross for CBB resistance by controlled greenhouse inoculations and visually assessed symptoms on days 7, 15, and 30 days after inoculation, using a scale where 0 = no disease and 5 = maximum susceptibility. Recent genetic mapping of an F 1 cross (TMS 30572 × CM 2177–2) led to the construction of the first molecular genetic map of cassava. Planting resistant varieties is the preferred method of disease control. manihotis ( Xam), is a major disease of cassava ( Manihot esculenta Crantz) in Africa and South America. Cassava bacterial blight (CBB), caused by Xanthomonas axonopodis pv. ![]()
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